Anti-HIR1 antibody (ab46878)
Key features and details
- Rabbit polyclonal to HIR1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-HIR1 antibody
See all HIR1 primary antibodies -
Description
Rabbit polyclonal to HIR1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Saccharomyces cerevisiae
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Saccharomyces cerevisiae HIR1.
Read Abcam's proprietary immunogen policy (Peptide available as ab61633.) -
Positive control
- This antibody gave a positive signal in Saccharomyces cerevisiae Yeast Extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Saccharomyces cerevisiaeApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).Target
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Relevance
HIR1 is a member of the HIR complex that also contains HIR2, HIR3 and HPC2. This complex is responsible for the repression of histone gene transcription as well as having a role in nucleosome assembly. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 852275 Saccharomyces cerevisiae
- SwissProt: P32479 Saccharomyces cerevisiae
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Alternative names
- Histone transcription regulator 1 antibody
- YBL008W antibody
Images
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Western blot - Anti-HIR1 antibody (ab46878)Anti-HIR1 antibody (ab46878) at 1 µg/ml + S Cerevisiae Yeast Extract at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 94 kDa
Additional bands at: 15 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (0)
ab46878 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HIR1 antibody (ab46878)Anti-HIR1 antibody (ab46878) at 1 µg/ml + S Cerevisiae Yeast Extract at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 94 kDa
Additional bands at: 15 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
