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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-HIR1 antibody (ab61635)

Anti-HIR1 antibody (ab61635)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to HIR1
  • Suitable for: WB
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG

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Overview

  • Product name

    Anti-HIR1 antibody
    See all HIR1 primary antibodies
  • Description

    Rabbit polyclonal to HIR1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Saccharomyces cerevisiae
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae HIR1 aa 500-600 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab61634)

  • Positive control

    • This antibody gave a positive signal in Saccharomyces cerevisiae Yeast Extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab61635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Saccharomyces cerevisiae
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).

Target

  • Relevance

    HIR1 is a member of the HIR complex that also contains HIR2, HIR3 and HPC2. This complex is responsible for the repression of histone gene transcription as well as having a role in nucleosome assembly.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 852275 Saccharomyces cerevisiae
    • SwissProt: P32479 Saccharomyces cerevisiae
    • Alternative names

      • Histone transcription regulator 1 antibody
      • YBL008W antibody

    Images

    • Western blot - Anti-HIR1 antibody (ab61635)
      Western blot - Anti-HIR1 antibody (ab61635)
      Anti-HIR1 antibody (ab61635) at 1 µg/ml + S cerevisiae Yeast Extract at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 94 kDa
      Observed band size: 94 kDa
      Additional bands at: 15 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab61635? Please let us know so that we can cite the reference in this datasheet.

    ab61635 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-HIR1 antibody (ab61635)
      Western blot - Anti-HIR1 antibody (ab61635)
      Anti-HIR1 antibody (ab61635) at 1 µg/ml + S cerevisiae Yeast Extract at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 94 kDa
      Observed band size: 94 kDa
      Additional bands at: 15 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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