Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3981] to MELK - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MELK antibody [EPR3981] - BSA and Azide free
    See all MELK primary antibodies

  • Description

    Rabbit monoclonal [EPR3981] to MELK - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HCT116, HAP1, HeLa, K562, Jurkat and HEK-293T cell lysates.

  • General notes

    ab172889 is the carrier-free version of ab108529 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab172889 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)
    Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)
    All lanes : Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution

    Lane 1 : HCT116 cell lysate
    Lane 2 : MELK knockout HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 75 kDa
    Observed band size: 75 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab108529).

    Lanes 1 - 2: Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab108529 was shown to react with MELK in wild-type HCT116 cells in western blot with loss of signal observed in  MELK knockout cell line ab266896 (MELK knockout cell lysate ab257537). Wild-type and  MELK knockout HCT116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108529 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)
    Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)

    This data was developed using ab108529, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: MELK knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: HCT116 cell lysate (20 µg)

    Lanes 1 - 4 Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab108529 was shown to recognize MELK when MELK knockout samples were used, along with additional cross-reactive bands. Wild-type and MELK knockout samples were subjected to SDS-PAGE. ab108529 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)
    Western blot - Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)
    All lanes : Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 75 kDa



    This data was developed using ab108529, the same antibody clone in a different buffer formulation.

     

  • Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)
    Anti-MELK antibody [EPR3981] - BSA and Azide free (ab172889)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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