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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing

Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)

Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15228] to PRPF8/Prp8 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free
    See all PRPF8/Prp8 primary antibodies
  • Description

    Rabbit monoclonal [EPR15228] to PRPF8/Prp8 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PRPF8/Prp8 aa 200-300. The exact sequence is proprietary.
    Database link: Q6P2Q9

  • General notes

    Ab251012 is the carrier-free version of ab190347. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251012 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15228
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Neuroscience
    • Sensory System
    • Visual system

Images

  • Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    All lanes : Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa
    Observed band size: 274 kDa



    This data was developed using ab190347, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/20000 dilution + 293T cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa
    Observed band size: 274 kDa



    This data was developed using ab190347, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    All lanes : Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/1000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa
    Observed band size: 274 kDa



    This data was developed using ab190347, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    This data was developed using ab190347, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human ovarian carcinoma tissue labeling PRPF8/Prp8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    This data was developed using ab190347, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling PRPF8/Prp8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)
    Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free (ab251012)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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