Anti-SRSF5 antibody (ab67175)
Key features and details
- Mouse polyclonal to SRSF5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SRSF5 antibody
See all SRSF5 primary antibodies -
Description
Mouse polyclonal to SRSF5 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human SRSF5.
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Positive control
- Human liver tissue lysate. Human ovary tissue sections. Hela cells. SRSF5 transfected 293T cell lysate
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General notes
Previously labelled as SFRS5.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SRSF5 antibody (ab67175)Anti-SRSF5 antibody (ab67175) at 1/500 dilution + human liver tissue lysate at 25 µg/ml
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
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Western blot - Anti-SRSF5 antibody (ab67175)All lanes : Anti-SRSF5 antibody (ab67175) at 1/500 dilution
Lane 1 : SRSF5 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF5 antibody (ab67175)ab67175, at 1.5µg/ml, staining SRSF5 in human ovary by immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-SRSF5 antibody (ab67175)ICC/IF image of ab67175 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67175, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
