Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTBP1 antibody [EPR9049(B)] (ab134950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9049(B)] to PTBP1
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PTBP1 antibody [EPR9049(B)]
    See all PTBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9049(B)] to PTBP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PTBP1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • WB: HAP1, HeLa, Daudi, Jurkat and BXPC3 cell lysates. ICC/IF: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    Immunofluorescent analysis of HeLa cells labelling PTBP1 with ab134950 at 1/100 dilution.
  • Western blot - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    Western blot - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PTBP1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab134950 was shown to react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265155 (knockout cell lysate ab257614) was used. Wild-type HeLa and PTBP1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab134950 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    Western blot - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PTBP1 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 57 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab134950 was shown to specifically react with PTBP1 in wild-type HAP1 cells as signal was lost in PTBP1 knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. ab134950 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    Western blot - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : BXPC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa

  • OI-RD Scanning - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    OI-RD Scanning - Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PTBP1 antibody [EPR9049(B)] (ab134950)
    Anti-PTBP1 antibody [EPR9049(B)] (ab134950)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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