Anti-CDC5L antibody [EPR7658] (ab129114)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7658] to CDC5L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-CDC5L antibody [EPR7658]
See all CDC5L primary antibodies -
Description
Rabbit monoclonal [EPR7658] to CDC5L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to residues in Human CDC5L (Q99459).
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Positive control
- Human kidney tissue; HepG2 cells; fetal brain and HeLa whole cell lysate (ab150035).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7658 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CDC5L antibody [EPR7658] (ab129114) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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ab129114, at a 1/250 dilution, staining CDC5L in HepG2 cells by immunofluorescence.
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ab129114, at a 1/250 dilution, staining CDC5L in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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