Anti-PTBP1 antibody (ab5642)
Key features and details
- Goat polyclonal to PTBP1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PTBP1 antibody
See all PTBP1 primary antibodies -
Description
Goat polyclonal to PTBP1 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Dog, Pig
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Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PTBP1 antibody (ab5642)All lanes : Anti-PTBP1 antibody (ab5642) at 0.01 µg/ml
Lane 1 : Human epithelial cells from cervix adenocarcinoma
Lane 2 : Human liver hepatocellular carcinoma
Lane 3 : Human epithelial cells from embryonic kidney
Lysates/proteins at 35 µg per lane.
Predicted band size: 61-64 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling PTBP1 with ab5642 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
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Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody (ab5642)ICC/IF image of ab5642 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5642, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)IHC image of ab5642 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5642, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-PTBP1 antibody (ab5642)Anti-PTBP1 antibody (ab5642) at 0.1 µg/ml + Human Ovary Lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 61-64 kDa
