Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22944-66] to MARCO - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
  • Reacts with: Mouse

Overview

  • Product name

    Anti-MARCO antibody [EPR22944-66] - BSA and Azide free
    See all MARCO primary antibodies

  • Description

    Rabbit monoclonal [EPR22944-66] to MARCO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: J774A.1 treated with 1ug/ml Lipopolysaccharides, Mouse spleen and Mouse spleen treated with Peptide:N-glycosidase F lysates. IHC-P: Mouse spleen and Mouse liver tissues. ICC/IF: J744A.1 cells.

  • General notes

    Ab259266 is the carrier-free version of ab259264. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab259266 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MARCO with ab259264 at 1/1000 dilution (0.501 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of mouse liver. The section was incubated with ab259264 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MARCO with ab259264 at 1/1000 dilution (0.501 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on macrophages of mouse spleen (PMID: 12874264, 15494482). The section was incubated with ab259264 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259264).

  • Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)
    Immunohistochemistry (Frozen sections) - Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)

    4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse spleen tissue labeling MARCO using ab259264 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on mouse splenic marginal zone (PMID: 24670793).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259264).

  • Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)
    Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J744A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cells labelling MARCO with ab259264 at 1/50 (10 ug/ml) dilution, followed by Ab259264 anti-MARCO ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). UNABLE TO ENCODE THE VARIABLE PLEASE DO MANUALLY is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Ab259264 anti-MARCO ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab259264).

  • Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)
    Anti-MARCO antibody [EPR22944-66] - BSA and Azide free (ab259266)

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