Anti-Cre recombinase antibody (ab190177)
Key features and details
- Rabbit polyclonal to Cre recombinase
- Suitable for: WB, ICC/IF
- Reacts with: Bacteriophage P1
- Isotype: IgG
Overview
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Product name
Anti-Cre recombinase antibody
See all Cre recombinase primary antibodies -
Description
Rabbit polyclonal to Cre recombinase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Bacteriophage P1 -
Immunogen
Full length protein corresponding to Bacteriophage P1 Cre recombinase aa 1 to the C-terminus. The immunogen was tagged with a 6xHis tag and an S tag.
Database link: P06956 -
Positive control
- WB: Human HEK-293T cells transfected with GFP-Cre recombinase cell lysate. ICC/IF: U-2 OS cells transfected with GFP-Cre recombinase.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cre recombinase antibody (ab190177) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T cells transfected with GFP-Cre recombinase lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 39 kDaPredicted band size : 26 kDa (GFP) 38 kDa (Cre)= 64 kDa
WB: 40 µg of whole cell extracts transfer in nitrocelullose, block: 1 hour 5% milk in TBST, incubation with primary antibody o/n @ +4°C in 5% milk TBST.
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Immunofluorescent analysis of U-2 OS cells (Human bone osteosarcoma epithelial cell line) transfected with GFP-Cre recombinase (green) labeling Cre recombinase with ab190177 at 1/3000 dilution (red). DAPI staining is shown in blue.