Anti-Cre recombinase antibody [7.23] (ab24607)
![Anti-Cre recombinase antibody [7.23] (ab24607)](https://www.abcam.com/ps/products/24/ab24607/Images/ab24607-35417-anti-cre-recombinase-antibody-723-western-blot.jpg "Anti-Cre recombinase antibody [7.23] (ab24607)")
Key features and details
- Mouse monoclonal [7.23] to Cre recombinase
- Suitable for: WB
- Isotype: IgG1
Overview
-
Product name
Anti-Cre recombinase antibody [7.23]
See all Cre recombinase primary antibodies -
Description
Mouse monoclonal [7.23] to Cre recombinase -
Host species
Mouse -
Specificity
Current Batch not validated for Immunohistochemistry. -
Tested applications
Suitable for: WBmore details -
Immunogen
Full length native protein (affinity purified).
-
General notes
This antibody can be used to determine the success of gene manipulations in transgenic animals.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
This antibody can be used to determine the success of gene manipulations in transgenic animals. -
Clonality
Monoclonal -
Clone number
7.23 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-Cre recombinase antibody [7.23] (ab24607) This image is courtesy of Britta Eickholt, Univ London Kings Coll, United KingdomAll lanes : Anti-Cre recombinase antibody [7.23] (ab24607) at 1/1000 dilution
Lane 1 : Escherichia coli expressing Cre-recombinase
Lane 2 : Hek cell expressing control plasmid
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-mouse HRP conjugated at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50&80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
