All lanes : Anti-SPHK1 antibody (ab260073) at 0.5 µg/ml

Lane 1 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 3 : Hut cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 43 kDa

Human placenta tissue stained for SPHK1 using ab260073 at 1 µg/ml in immunohistochemistry. Heat mediated antigen retrieval was performed.

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SPHK1 using ab260073 at 1 µg/ml in ICC.

Flow Cytometry analysis of U87 (human glioblastoma-astrocytoma epithelial cell line) cells using ab260073 (Blue line).
The cells were blocked with 10% normal goat serum and then incubated with ab260073 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabeled sample (Red line) was also used as a control.

Flow Cytometry analysis of U-2 0S (human bone osteosarcoma epithelial cell line) cells using ab260073 (Blue line).
The cells were blocked with 10% normal goat serum and then incubated with ab260073 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabeled sample (Red line) was also used as a control.