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Anti-SPHK1 antibody [EPR4293(2)] - BSA and Azide free (ab247890)

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  • Western blot - Anti-SPHK1 antibody [EPR4293(2)] - BSA and Azide free (ab247890)
    Western blot - Anti-SPHK1 antibody [EPR4293(2)] - BSA and Azide free (ab247890)
    All lanes : Anti-SPHK1 antibody [EPR4293(2)] (ab109522) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : HT-1080 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 43 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109522, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-SPHK1 antibody [EPR4293(2)] - BSA and Azide free (ab247890)
    Flow Cytometry - Anti-SPHK1 antibody [EPR4293(2)] - BSA and Azide free (ab247890)
    This data was developed using ab109522, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Raji cells using ab109522 (red), or a rabbit IgG (negative) (green).
  • Anti-SPHK1 antibody [EPR4293(2)] - BSA and Azide free (ab247890)
    Anti-SPHK1 antibody [EPR4293(2)] - BSA and Azide free (ab247890)

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