Anti-SPHK1 antibody (ab262697)
Key features and details
- Rabbit polyclonal to SPHK1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPHK1 antibody
See all SPHK1 primary antibodies -
Description
Rabbit polyclonal to SPHK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SPHK1 (C terminal). The exact sequence is proprietary. Carrier protein conjugated.
Database link: Q9NYA1 -
Positive control
- WB: Molt-4 whole cell lysate; ICC/IF: A431 cells; IHC-P: A549 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPHK1 antibody (ab262697)
Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue staining SPHK1 with ab262697 at 1/100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15mins.
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Western blot - Anti-SPHK1 antibody (ab262697)Anti-SPHK1 antibody (ab262697) at 1/500 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 43 kDa10% SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-SPHK1 antibody (ab262697)Immunocytochemistry/Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling SPHK1 with ab262697 at 1/200 dilution.
