Anti-SPHK1 antibody [EPR4293(2)] (ab109522)
![Anti-SPHK1 antibody [EPR4293(2)] (ab109522)](https://www.abcam.com/ps/products/109/ab109522/Images/ab109522-122446-anti-sphk1-antibody-epr42932-western-blot.jpg "Anti-SPHK1 antibody [EPR4293(2)] (ab109522)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4293(2)] to SPHK1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-SPHK1 antibody [EPR4293(2)]
See all SPHK1 primary antibodies -
Description
Rabbit monoclonal [EPR4293(2)] to SPHK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji, JAR, HUVEC or HT-1080 lysate Permeabilized Raji cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4293(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPHK1 antibody [EPR4293(2)] (ab109522)All lanes : Anti-SPHK1 antibody [EPR4293(2)] (ab109522) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : JAR cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-SPHK1 antibody [EPR4293(2)] (ab109522)](https://www.abcam.com/ps/products/109/ab109522/Images/ab109522-122445-anti-sphk1-antibody-epr42932-flow-cytometry.jpg)
Flow Cytometry - Anti-SPHK1 antibody [EPR4293(2)] (ab109522)Flow cytometric analysis of permeabilized Raji cells using ab109522 (red), or a rabbit IgG (negative) (green). -
![Anti-SPHK1 antibody [EPR4293(2)] (ab109522)](https://www.abcam.com/ps/products/109/ab109522/Images/ab109522-3-benefits-of-recombinant-antibodies.png)
Anti-SPHK1 antibody [EPR4293(2)] (ab109522)
