Anti-SPHK1 antibody (ab228645)
Key features and details
- Rabbit polyclonal to SPHK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPHK1 antibody
See all SPHK1 primary antibodies -
Description
Rabbit polyclonal to SPHK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human SPHK1 (internal sequence). The exact sequence is proprietary.
Database link: Q9NYA1 -
Positive control
- WB: MOLT-4 whole cell lysate. ICC/IF: A-375 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPHK1 antibody (ab228645)Anti-SPHK1 antibody (ab228645) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 43 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-SPHK1 antibody (ab228645)4% paraformaldehyde-fixed A-375 (human malignant melanoma cell line) cells stained for SPHK1 (green) using ab228645 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
