Anti-SPHK1 antibody (ab245354)
Key features and details
- Rabbit polyclonal to SPHK1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPHK1 antibody
See all SPHK1 primary antibodies -
Description
Rabbit polyclonal to SPHK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human SPHK1 aa 334-384. The exact sequence is proprietary. (NP_001136073.1).
Database link: Q9NYA1 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245354 was affinity purified using an epitope specific to SPHK1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-SPHK1 antibody (ab245354)
SPHK1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245354 at 10 μg/ml. Western blot was performed on the immunoprecipitate using ab245354 at 1 μg/ml.
Lane 1: ab245354 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence at an exposure time of 30 seconds.
The more slowly migrating bands are believed to represent additional forms of SPHK1 owing to recognition by multiple antibodies each of which was directed toward distinctly different epitopes on SPHK1.
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Western blot - Anti-SPHK1 antibody (ab245354)All lanes : Anti-SPHK1 antibody (ab245354) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 30 secondsThe more slowly migrating bands are believed to represent additional forms of SPHK1 owing to recognition by multiple antibodies each of which was directed toward distinctly different epitopes on SPHK1.
