Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PIP5K1C antibody [MAO-R1] - BSA and Azide free (ab247787)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MAO-R1] to PIP5K1C - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PIP5K1C antibody [MAO-R1] - BSA and Azide free
    See all PIP5K1C primary antibodies

  • Description

    Rabbit monoclonal [MAO-R1] to PIP5K1C - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length protein corresponding to Mouse PIP5K1C.

  • Positive control

    • WB: HeLa, Neuro-2a, K562, C6 and 293T cell lysates; Human fetal kidney tissue lysate.

  • General notes

    ab247787 is the carrier-free version of ab109192 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247787 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PIP5KI gamma

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    MAO-R1

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PIP5K1C antibody [MAO-R1] - BSA and Azide free (ab247787)
    Western blot - Anti-PIP5K1C antibody [MAO-R1] - BSA and Azide free (ab247787)
    All lanes : Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PIP5K1C knockout HeLa cell lysate
    Lane 3 : Neuro-2a cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

    Predicted band size: 73 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab109192 observed at 73 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab109192 Anti-PIP5K1C antibody [MAO-R1] was shown to specifically react with PIP5K1C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265545 (knockout cell lysate ab258117) was used. Wild-type and PIP5K1C knockout samples were subjected to SDS-PAGE. ab109192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PIP5K1C antibody [MAO-R1] - BSA and Azide free (ab247787)
    Western blot - Anti-PIP5K1C antibody [MAO-R1] - BSA and Azide free (ab247787)
    All lanes : Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Human fetal kidney cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : Neuro-2a cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 73 kDa



    This data was developed using ab109192, the same antibody clone in a different buffer formulation.

     

  • Anti-PIP5K1C antibody [MAO-R1] - BSA and Azide free (ab247787)
    Anti-PIP5K1C antibody [MAO-R1] - BSA and Azide free (ab247787)

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