Anti-HDLBP antibody [EPR5267] (ab109324)
![Anti-HDLBP antibody [EPR5267] (ab109324)](https://www.abcam.com/ps/products/109/ab109324/Images/ab109324-122105-anti-hdlbp-antibody-epr5267-western-blot.jpg "Anti-HDLBP antibody [EPR5267] (ab109324)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5267] to HDLBP
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-HDLBP antibody [EPR5267]
See all HDLBP primary antibodies -
Description
Rabbit monoclonal [EPR5267] to HDLBP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, Jurkat, HeLa and HepG2 cell lysates IHC-P: Human stomach tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5267 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HDLBP antibody [EPR5267] (ab109324)All lanes : Anti-HDLBP antibody [EPR5267] (ab109324) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 141 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDLBP antibody [EPR5267] (ab109324)](https://www.abcam.com/ps/products/109/ab109324/Images/ab109324-122104-anti-hdlbp-antibody-epr5267-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDLBP antibody [EPR5267] (ab109324)ab109324, at a 1/100 dilution, staining HDLBP in paraffin embedded Human stomach tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-HDLBP antibody [EPR5267] (ab109324)](https://www.abcam.com/ps/products/109/ab109324/Images/ab109324-4-benefits-of-recombinant-antibodies.png)
Anti-HDLBP antibody [EPR5267] (ab109324)
