Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11912(B)] to IGFBP7
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-IGFBP7 antibody [EPR11912(B)]
See all IGFBP7 primary antibodies
Description

Rabbit monoclonal [EPR11912(B)] to IGFBP7
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human IGFBP7 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q16270
Positive control
- SW480, 293T and MCF7 cell lysates; Human pancreas, prostate and lung cancer tissues; MCF7 cells; 293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11912(B)
Isotype

IgG
Research areas

Western blot - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

All lanes : Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/1000 dilution

Lane 1 : SW480 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 29 kDa

This image was produced using unpurified antibody.
Immunocytochemistry/ Immunofluorescence - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with Purified ab171085 at 1:100 (6.0 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with purified ab171085 at 1:60 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IGFBP7 with purified ab171085 at 1:250 dilution (2.4 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/10000 dilution (Purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 29 kDa
Observed band size: 29 kDa
Flow Cytometry - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Flow cytometric analysis of permeabilized 293T cells labeling IGFBP7 with ab171085 at 1/250 dilution (red) compared to a rabbit IgG negative control (green). This image was produced using unpurified antibody.
Immunocytochemistry/ Immunofluorescence - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Immunofluorescent analysis of MCF7 cells labeling IGFBP7 with ab171085 at 1/250 dilution. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.
Anti-IGFBP7 antibody [EPR11912(B)] (ab171085)

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