Anti-IGFBP7 antibody (ab36661)
Key features and details
- Rabbit polyclonal to IGFBP7
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IGFBP7 antibody
See all IGFBP7 primary antibodies -
Description
Rabbit polyclonal to IGFBP7 -
Host species
Rabbit -
Specificity
This antibody reacts with IGFBP7. It does not recognize IGFBPs 1,2,3,4,5,or 6. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Epitope of human IGFBP7.
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General notes
Dissolved in 400 µl sterile water (a 1/20 dilution). Dilute further in a buffer solution appropriate for the desired application.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody is affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody (ab36661)ab36661 (4µg/ml) staining IGFBP7 in human renal cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of renal tubules.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
