Overlay histogram showing SHSY-5Y cells stained with ab109630 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109630, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-LIS1 antibody [EPR3335(2)] (ab109630) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 47 kDa

Flow cytometric analysis of permeabilized HeLa cells using ab109630 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).