Anti-LIS1 antibody (ab2607)
Key features and details
- Rabbit polyclonal to LIS1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-LIS1 antibody
See all LIS1 primary antibodies -
Description
Rabbit polyclonal to LIS1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
-
Immunogen
Synthetic peptide corresponding to Human LIS1. Represents a portion of human Lissencephaly 1 (Lis 1) protein encoded in part by exons 3 and 4.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purification notes
Affinity purified using the immunising peptideimmobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-LIS1 antibody (ab2607)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIS1 antibody (ab2607)ab2607 (2µg/ml) staining Lis1 in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic compartment of the mature spermatocyte cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
