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Neuroscience Neurology process Neural Signal Transduction

Anti-Gemin 3 antibody [EPR11283] (ab166913)

Price and availability

298 185 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Gemin 3 antibody [EPR11283] (ab166913)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11283] to Gemin 3
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-Gemin 3 antibody [EPR11283]
    See all Gemin 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR11283] to Gemin 3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Gemin 3. The exact sequence is proprietary.

  • Positive control

    • HeLa, MCF-7 and 293T cell lysate. Human colon tissue. Human kidney tissue. Permeabilized 293T cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11283
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Images

  • Western blot - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Western blot - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    All lanes : Anti-Gemin 3 antibody [EPR11283] (ab166913) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] (ab166913)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling Gemin 3 with purified ab166913 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] (ab166913)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Gemin 3 with ab166913 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] (ab166913)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Gemin 3 with ab166913 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Immunocytochemistry/ Immunofluorescence - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Immunofluorescent analysis of 293T cells labeling Gemin 3 with ab166913 at 1/100 dilution.
  • Flow Cytometry - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Flow Cytometry - Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Flow cytometric analysis of permeabilized 293T cells labeling Gemin 3 with ab166913 at 1/100 (red) or a rabbit IgG control (negative) (green).
  • Anti-Gemin 3 antibody [EPR11283] (ab166913)
    Anti-Gemin 3 antibody [EPR11283] (ab166913)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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