Anti-AKT1 (phospho T34) antibody (ab23509)
Key features and details
- Rabbit polyclonal to AKT1 (phospho T34)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKT1 (phospho T34) antibody
See all AKT1 primary antibodies -
Description
Rabbit polyclonal to AKT1 (phospho T34) -
Host species
Rabbit -
Specificity
The antibody detects a 56kDa protein corresponding to the apparent molecular mass of phoshorylated Akt on SDS-PAGE immunoblots of A431 plus calyculin A cell lysate. Similar results were seen in calyculin A treated human aortic endothelial and HeLa cells, rabbit spleen fibroblasts, and rat pituitary cells. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Phospho-Akt (Thr-34) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around threonine 34 of human Akt.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT1 (phospho T34) antibody (ab23509)All lanes : Anti-AKT1 (phospho T34) antibody (ab23509)
Lane 1 : A431 (Human epithelial carcinoma cell line)serum starved overnight
Lane 2 : A431 (Human epithelial carcinoma cell line)calyculin A (100nM) treated for 30mins
Lysates/proteins at 20 µg per lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
