Anti-14-3-3 antibody [EPR6379] - BSA and Azide free (ab248475)
![Anti-14-3-3 antibody [EPR6379] - BSA and Azide free (ab248475)](https://www.abcam.com/ps/products/248/ab248475/Images/ab248475-402578-anti-14-3-3-antibody-epr6379-bsa-and-azide-free-western-blot.jpg "Anti-14-3-3 antibody [EPR6379] - BSA and Azide free (ab248475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6379] to 14-3-3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-14-3-3 antibody [EPR6379] - BSA and Azide free
See all 14-3-3 primary antibodies -
Description
Rabbit monoclonal [EPR6379] to 14-3-3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248475 is the carrier-free version of ab133323. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248475 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.43 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6379 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 antibody [EPR6379] - BSA and Azide free (ab248475)All lanes : Anti-14-3-3 antibody [EPR6379] (ab133323) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : Raw264.7 cell lysate
Lane 7 : PC-12 cell lysate
Lane 8 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDaThis data was developed using ab133323, the same antibody clone in a different buffer formulation.
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![OI-RD Scanning - Anti-14-3-3 antibody [EPR6379] - BSA and Azide free (ab248475)](https://www.abcam.com/ps/products/248/ab248475/Images/ab248475-1-anti-14-3-3-antibody-epr6379-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-14-3-3 antibody [EPR6379] - BSA and Azide free (ab248475)This data was developed using ab133323, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-14-3-3 antibody [EPR6379] - BSA and Azide free (ab248475)](https://www.abcam.com/ps/products/248/ab248475/Images/ab248475-3-benefits-of-recombinant-antibodies.png)
Anti-14-3-3 antibody [EPR6379] - BSA and Azide free (ab248475)
