Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23564-282] to SOX6 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free
    See all SOX6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23564-282] to SOX6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human astrocytoma and skin tissue.

  • General notes

    ab277527 is the carrier-free version of ab245215. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)

    This data was developed using ab245215, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling SOX6 with ab245215 at 1/500 (0.892 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human astrocytoma (PMID: 14691456, PMID: 15696972). The section was incubated with ab245215 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)

    This data was developed using ab245215, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling SOX6 with ab245215 at 1/500 (0.892 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in basal cells of human skin (PMID: 32088305). The section was incubated with ab245215 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)

    This data was developed using ab245215, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling SOX6 with ab245215 at 1/500 (0.892 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human spleen (PMID: 14691456). The section was incubated with ab245215 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)
    Anti-SOX6 antibody [EPR23564-282] - BSA and Azide free (ab277527)

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