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Anti-Tau (phospho S199) antibody [EPR2401Y] - BSA and Azide free (ab166747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2401Y] to Tau (phospho S199) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tau (phospho S199) antibody [EPR2401Y] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR2401Y] to Tau (phospho S199) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab81268 only detects Tau phosphorylated on Serine 199.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse cerebral cortex tissue lysate.

  • General notes

    Ab166747 is the carrier-free version of ab81268. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab166747 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-Tau (phospho S199) antibody [EPR2401Y] - BSA and Azide free (ab166747)
    Dot Blot - Anti-Tau (phospho S199) antibody [EPR2401Y] - BSA and Azide free (ab166747)

    Dot blot analysis of Tau (pS199) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS199) with ab81268 at a dilution of 1/1000. ab97051 (peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Exposure time: 3 minutes.
    Blocking and dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81268).

  • Western blot - Anti-Tau (phospho S199) antibody [EPR2401Y] - BSA and Azide free (ab166747)
    Western blot - Anti-Tau (phospho S199) antibody [EPR2401Y] - BSA and Azide free (ab166747)
    All lanes : Anti-Tau (phospho S199) antibody [EPR2401Y] (ab81268) at 1/1000 dilution

    Lane 1 : Mouse cerebral cortex tissue lysate
    Lane 2 : Mouse cerebral cortex tissue lysate, The membrane was incubated with phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000000 dilution

    Predicted band size: 78 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81268).

  • Anti-Tau (phospho S199) antibody [EPR2401Y] - BSA and Azide free (ab166747)
    Anti-Tau (phospho S199) antibody [EPR2401Y] - BSA and Azide free (ab166747)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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