Anti-Ubiquitin antibody (ab223613)
Key features and details
- Rabbit polyclonal to Ubiquitin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Ubiquitin antibody
See all Ubiquitin primary antibodies -
Description
Rabbit polyclonal to Ubiquitin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to keyhole limpet haemocyanin.
Database link: P0CG53 -
Positive control
- WB: HEK-293 cell lysate. IHC-P: Mouse backskin tissue; Human colon carcinoma tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody (ab223613)
2% formaldehyde-fixed heat shocked (at 42°C for 1 hour) HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (yellow) using ab223613 at at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit R-PE at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature.
A) DAPI (blue) nuclear stain. (B) ab223613. (C) Merge.
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Western blot - Anti-Ubiquitin antibody (ab223613)Anti-Ubiquitin antibody (ab223613) at 1/1000 dilution + HEK-293 (human epithelial cell line from embryonic kidney)cell lysate
Predicted band size: 26 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab223613)Mouse backskin tissue tissue stained for Ubiquitin (green) using ab223613 at 1/100 dilution in immunohistochemical analysis, followed by Goat Anti-Rabbit FITC at 1/50 dilution for 1 hour at room temperature.
Fixation: Bouin’s Fixative Solution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (ab223613)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Ubiquitin using ab223613 at 1/100000 dilution in immunohistochemical analysis, followed by Goat Anti-Rabbit Biotin at 1/2000 dilution for 1 hour at room temperature.
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Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody (ab223613)2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (green) using ab223613 at 1/200 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature.
A) DAPI (blue) nuclear stain. (B) ab223613. (C) Merge.
