Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ubiquitin antibody [EPR8590] (ab137025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8590] to Ubiquitin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Ubiquitin antibody [EPR8590]
    See all Ubiquitin primary antibodies

  • Description

    Rabbit monoclonal [EPR8590] to Ubiquitin

  • Host species

    Rabbit

  • Specificity

    ab137025 recognizes Ubiquitin, polyubiquitin and ubiquitinated proteins.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Ubiquitin. The exact sequence is proprietary.

  • Positive control

    • Fetal brain, HepG2, Jurkat, and 293T cell lysates; Human breast carcinoma tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Ubiquitin antibody [EPR8590] (ab137025)
    Western blot - Anti-Ubiquitin antibody [EPR8590] (ab137025)
    All lanes : Anti-Ubiquitin antibody [EPR8590] (ab137025) at 1/200 dilution

    Lane 1 : MCF-7
    Lane 2 : MCF-7 Whole cell lysate + M132 (50 uM 90 min)
    Lane 3 : Mouse Brain

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 9 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. ab137025 and ab8245 (loading control to GAPDH) were diluted 1/200 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging  using the Licor Odyssey CLx.

  • Western blot - Anti-Ubiquitin antibody [EPR8590] (ab137025)
    Western blot - Anti-Ubiquitin antibody [EPR8590] (ab137025)
    All lanes : Anti-Ubiquitin antibody [EPR8590] (ab137025) at 1/1000 dilution

    Lane 1 : Fetal brain cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 9 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody [EPR8590] (ab137025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody [EPR8590] (ab137025)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Ubiquitin with ab137025 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Ubiquitin antibody [EPR8590] (ab137025)
    Anti-Ubiquitin antibody [EPR8590] (ab137025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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