Anti-Ubiquitin antibody [5B9-B3] (ab223378)
![Anti-Ubiquitin antibody [5B9-B3] (ab223378)](https://www.abcam.com/ps/products/223/ab223378/Images/ab223378-283123-ab223378ICC2.png "Anti-Ubiquitin antibody [5B9-B3] (ab223378)")
Key features and details
- Mouse monoclonal [5B9-B3] to Ubiquitin
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Ubiquitin antibody [5B9-B3]
See all Ubiquitin primary antibodies -
Description
Mouse monoclonal [5B9-B3] to Ubiquitin -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to keyhole limpet haemocyanin.
Database link: P0CG53 -
Positive control
- ICC/IF: HeLa cells. WB: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5B9-B3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [5B9-B3] (ab223378)
2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (green) using ab223378 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT.
(A) DAPI nuclear stain (B) ab223378 (C) Merge.
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![Western blot - Anti-Ubiquitin antibody [5B9-B3] (ab223378)](https://www.abcam.com/ps/products/223/ab223378/Images/ab223378-283121-ab223378WB.png)
Western blot - Anti-Ubiquitin antibody [5B9-B3] (ab223378)Anti-Ubiquitin antibody [5B9-B3] (ab223378) at 1/1000 dilution + Mix of human cell lysates at 15 µg
Secondary
Sheep Anti-Mouse IgG HRP
Predicted band size: 34 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?Blocking buffer: 1.5% BSA.
Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.
Mix of human cell line lysates: epidermoid carcinoma (A431), adenocarcinomic alveolar basal epithelial cells (A549), colon cancer (HCT116), cervical cancer (HeLa), embryonic kidney (HEK293), liver cancer (HepG2), promyelocytic leukemia (HL-60), umbilical vein endothelial cells (HUVEC), T lymphocytes (Jurkat), breast cancer (MCF7), prostate cancer (PC3), brain glioblastom (T98G).
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![Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [5B9-B3] (ab223378)](https://www.abcam.com/ps/products/223/ab223378/Images/ab223378-283122-ab223378ICC1.png)
Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [5B9-B3] (ab223378)2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (green) using ab223378 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Mouse FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT.
(A) DAPI nuclear stain (B) ab223378 (C) Merge.
