Ubn-ubiquitinylated substrate (ab157043)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, MS, Functional Studies, SDS-PAGE
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Product name
Ubn-ubiquitinylated substrate
See all Ubiquitin proteins and peptides -
Purity
> 95 % SDS-PAGE.
High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Additional sequence information
Synthesized enzymatically in vitro, produced using wild type ubiquitin. Uniprot ids: P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52). MW ~25-150 kDa
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Description
Recombinant human Ubiquitin protein
Preparation and Storage
- Epididymis secretory protein Li 50
- FLJ25987
- HEL S 50
Images
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Western blot - Ubn-ubiquitinylated substrate (ab157043)Lane 1 : MW Marker
Lanes 2-3 : Ubiquitin linkage-specific mAb
Lane 2 : Substrate treated with USP2
Lane 3 : Substrate without protease
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SDS-PAGE - Ubn-ubiquitinylated substrate (ab157043)Lane 1: MW Marker, Lane 2: 2 μg of ab157043, Lane 3: 5 μg of ab157043, Lane 4: 10 μg of ab157043
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SDS-PAGE - Ubn-ubiquitinylated substrate (ab157043)DUB assay. ab157043 was incubated with USP2. Lane 1: MW Marker, Lane 2: Substrate without protease, Lane 3: Substrate with protease, Lane 4: Protease without substrate.
