Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27)
Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
See all Ubiquitin primary antibodies
Description

Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K27-linked-Ub2 recombinant protein. C6, RAW 264.7, PC-12, NIH/3T3, HeLa, 293T and Jurkat whole cell lysates. Rat and Mouse brain, heart, kidney and spleen lysates. IHC-P: Human transitional cell carcinoma of bladder tissue. Mouse and Rat spleen tissue. Flow Cytometry: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17034
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma)
labelling Ubiquitin (linkage-specific K27) with purified ab181537 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

All lanes : Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution

Lane 1 : K27-linked-Ub2 recombinant protein
Lane 2 : K6-linked-Ub2 recombinant protein
Lane 3 : K11-linked-Ub2 recombinant protein
Lane 4 : K29-linked-Ub2 recombinant protein
Lane 5 : K33-linked-Ub2 recombinant protein
Lane 6 : K48-linked-Ub2 recombinant protein
Lane 7 : K63-linked-Ub2 recombinant protein
Lane 8 : Ubiquitin recombinant protein
Lane 9 : Ubiquitin linear recombinant protein

Lysates/proteins at 0.02 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Blocking/dilution buffer: 5% BSA/TBST.
Expected MW is ~17kDa (8.5*2)
The ~30kDa band may be the dimer of K27-linked-Ub2 recombinant protein.
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

All lanes : Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/50000 dilution

Lane 1 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Observed band size: 14 & above kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% BSA/TBST.
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Observed band size: 14 & above kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% BSA/TBST.
Western blot - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

All lanes : Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lane 7 : Rat kidney lysates
Lane 8 : Rat spleen lysates
Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Observed band size: 14 & above kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% BSA/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on cancer cells of Human bladder transitional cell carcinoma is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Mouse spleen is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Rat spleen is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/160 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and cell without incubation with primary antibody and secondary antibody is blue.
Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

Alternative products to Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)

Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237255)

Anti-Ubiquitin antibody [EP296Y] (ab33893)

Alexa Fluor® 488 Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237254)

Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)

Alexa Fluor® 647 Anti-Ubiquitin antibody [EPR8830] (ab205468)

Anti-Ubiquitin antibody [Ubi-1] (ab7254)

Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K48) antibody [EP8589] (ab204476)

Anti-Ubiquitin (linkage-specific K48) antibody [EP8589] - BSA and Azide free (ab271911)