Anti-Ubiquitin antibody (ab19247)
Key features and details
- Rabbit polyclonal to Ubiquitin
- Suitable for: WB
- Reacts with: Mouse, Human, Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Ubiquitin antibody
See all Ubiquitin primary antibodies -
Description
Rabbit polyclonal to Ubiquitin -
Host species
Rabbit -
Specificity
It can identify free ubiquitin as well as ubiquinated proteins. The antibody recognizes polyubiquitin chains more strongly than monoubiquitinated molecules. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human, Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to Keyhole Limpet Haemocyanin (KLH).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ubiquitin antibody (ab19247)All lanes :
Lane 1 : HeLa cell lysate (Control, untreated)
Lane 2 : HeLa cells treated with MG132 (50 uM for 90 min)
Lane 3 : Mouse Brain
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 10 kDaThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. ab19247 and ab8245 (loading control to GAPDH) were diluted 1/200 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging using the Licor Odyssey CLx.
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Western blot - Anti-Ubiquitin antibody (ab19247)Anti-Ubiquitin antibody (ab19247) at 1/1000 dilution + Heat-shocked HeLa cell lysate
Predicted band size: 10 kDa
