Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22064] to Tau (phospho T181) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR22064] to Tau (phospho T181) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain lysate.

  • General notes

    Ab240579 is the carrier-free version of ab223192. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240579 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)
    Western blot - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)
    All lanes : Anti-Tau (phospho T181) antibody [EPR22064] (ab223192) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate (WB membrane treated with alkaline phosphatase)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    WB membrane in Lane 2 was treated with alkaline phosphatase for 1h at 37°C.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223192).

  • Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)
    Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)

    Tau (phospho T181 was immunoprecipitated from 0.35 mg of human brain lysate with ab223192 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223192 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human brain lysate 10 μg (Input).
    Lane 2: ab223192 IP in Human brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223192 in Human brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223192).

  • Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)
    Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)

    Dot blot analysis of Tau (phospho T181) labeled with ab223192 at 1/1000 dilution.

    Lane 1: Tau (phospho T181) peptide.
    Lane 2: Tau non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used for detection at 1/100000 dilution.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223192).

  • Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)
    Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (ab240579)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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