Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18229] to VE Cadherin - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free
    See all VE Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR18229] to VE Cadherin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: bEnd.3 cells.

  • General notes

    Ab232515 is the carrier-free version of ab205336. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232515 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
    Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)

    VE Cadherin was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab205336 at 1/80 dilution.

    Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: Mouse lung whole cell lysate 10ug (Input).

    Lane 2: ab205336 IP in Mouse lung whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in Mouse lung whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

     

    Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).

  • Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
    Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)

    VE Cadherin was immunoprecipitated from 1mg of bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate with ab205336 at 1/80 dilution.

    Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: bEnd.3 whole cell lysate 10ug (Input).

    Lane 2: ab205336 IP in bEnd.3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in bEnd.3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).

  • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
    Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (Mouse brain microvascular endothelial cell line) cells labeling VE Cadherin with ab205336 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing membrane staining on bEnd.3 cell line. 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody -Loading control (ab7291) at  1/1000 dilution and Goat Anti-Mouse IgG  H&L  (AlexaFluor®594 ) preadsorbed (ab150120) at 1/500 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab205336 at 1/1000 dilution followed by ab150120 at 1/500 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).

  • Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
    Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)

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