Anti-VE Cadherin (phospho Y685) antibody (ab119785)
Key features and details
- Rabbit polyclonal to VE Cadherin (phospho Y685)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VE Cadherin (phospho Y685) antibody
See all VE Cadherin primary antibodies -
Description
Rabbit polyclonal to VE Cadherin (phospho Y685) -
Host species
Rabbit -
Specificity
ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho-peptide (Y685), conjugated to carrier protein, corresponding to amino acids surrounding tyrosine 685 in Human VE Cadherin.
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Positive control
- Human umbilical vein endothelial cells stimulated with pervanadate.
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General notes
Do not aliquot.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab119785 was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE Cadherin (Y685) peptide before affinity purification using phospho-VE Cadherin (Y685) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VE Cadherin (phospho Y685) antibody (ab119785)All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution
Lane 1 : Human umbilical vein endothelial
cells stimulated with pervanadate (1 mM) for 30 min.
Lane 2 : Human umbilical vein endothelial
cells stimulated with pervanadate (1 mM) for 30 min then treated with
alkaline phosphatase
Predicted band size: 88 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
