Anti-Flavivirus E-glycoprotein antibody [HF8-3/D5-10/6/A6] (ab214336)
Key features and details
- Mouse monoclonal [HF8-3/D5-10/6/A6] to Flavivirus E-glycoprotein
- Suitable for: Sandwich ELISA, WB, ICC/IF
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
- Isotype: IgG1
Overview
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Product name
Anti-Flavivirus E-glycoprotein antibody [HF8-3/D5-10/6/A6]
See all Flavivirus E-glycoprotein primary antibodies -
Description
Mouse monoclonal [HF8-3/D5-10/6/A6] to Flavivirus E-glycoprotein -
Host species
Mouse -
Specificity
ab214336 reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV. -
Tested applications
Suitable for: Sandwich ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus -
Immunogen
Recombinant full length protein corresponding to Dengue virus 1 Flavivirus E-glycoprotein aa 250-800.
Database link: P27909 -
Positive control
- Sandwich ELISA: Zika infected C6/36 cells; WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4, ZIKV infected cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
HF8-3/D5-10/6/A6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Sandwich ELISA detection Flavivirus E-glycoprotein in Zika infected and non infected C6/36 cells using ab214336 and other anti Flavivirus E-glycoprotein antibodies as capture antibody.
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All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-3/D5-10/6/A6] (ab214336)
Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV3 (H89) infected C6/36 cell lysate
Lane 4 : DENV4 (H241) infected C6/36 cell lysate
Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : YFV (17D) infected C6/36 cell lysate
Lane 8 : WNV (E101) infected C6/36 cell lysate
Lane 9 : uninfected C6/36 cell lysate control
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Immunofluorescent analysis of DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214336.