Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

Key features and details

  • Mouse monoclonal [HF8-1/C1-6/E12] to Flavivirus E-glycoprotein
  • Suitable for: WB, ICC/IF, ELISA, Sandwich ELISA
  • Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
  • Isotype: IgG1

Overview

  • Product name

    Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12]
    See all Flavivirus E-glycoprotein primary antibodies

  • Description

    Mouse monoclonal [HF8-1/C1-6/E12] to Flavivirus E-glycoprotein

  • Host species

    Mouse

  • Specificity

    ab214335 reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV.

  • Tested applications

    Suitable for: WB, ICC/IF, ELISA, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus

  • Immunogen

    Recombinant full length protein corresponding to Dengue virus 1 Flavivirus E-glycoprotein aa 250-800.
    Database link: P27909

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4, ZIKV infected cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Proclin 150
    Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    HF8-1/C1-6/E12

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
    Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

    Immunofluorescent analysis of DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214335.

  • ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
    ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

    ELISA analysis using Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

  • Sandwich ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
    Sandwich ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

    Sandwich ELISA detection Flavivirus E-glycoprotein in Zika infected and non infected C6/36 cells using ab214335 and other anti Flavivirus E-glycoprotein antibodies as capture antibody. 

  • Western blot - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
    Western blot - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
    All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

    Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
    Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
    Lane 3 : DENV3 (H89) infected C6/36 cell lysate
    Lane 4 : DENV4 (H241) infected C6/36 cell lysate
    Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
    Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
    Lane 7 : YFV (17D) infected C6/36 cell lysate
    Lane 8 : WNV (E101) infected C6/36 cell lysate
    Lane 9 : C6/36 cell lysate control

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