Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

1 ml 278 083 ₸

Order now and get it on Tuesday August 03, 2021

Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

Key features and details

Mouse monoclonal [HF8-1/C1-6/E12] to Flavivirus E-glycoprotein
Suitable for: WB, ICC/IF, ELISA, Sandwich ELISA
Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
Isotype: IgG1

Product name

Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12]
See all Flavivirus E-glycoprotein primary antibodies
Description

Mouse monoclonal [HF8-1/C1-6/E12] to Flavivirus E-glycoprotein
Host species

Mouse
Specificity

ab214335 reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV.
Tested applications

Suitable for: WB, ICC/IF, ELISA, Sandwich ELISAmore details
Species reactivity

Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
Immunogen

Recombinant full length protein corresponding to Dengue virus 1 Flavivirus E-glycoprotein aa 250-800.
Database link: P27909

Run BLAST with Run BLAST with
Positive control
- WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4, ZIKV infected cells
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.1% Proclin 150
Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

HF8-1/C1-6/E12
Isotype

IgG1
Light chain type

kappa
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

Immunofluorescent analysis of DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214335.
ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

ELISA analysis using Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
Sandwich ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

Sandwich ELISA detection Flavivirus E-glycoprotein in Zika infected and non infected C6/36 cells using ab214335 and other anti Flavivirus E-glycoprotein antibodies as capture antibody.
Western blot - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)

Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV3 (H89) infected C6/36 cell lysate
Lane 4 : DENV4 (H241) infected C6/36 cell lysate
Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : YFV (17D) infected C6/36 cell lysate
Lane 8 : WNV (E101) infected C6/36 cell lysate
Lane 9 : C6/36 cell lysate control

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