Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

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Anti-VE Cadherin antibody [EPR18229] (ab205336)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18229] to VE Cadherin
Suitable for: WB, ICC/IF, IP
Reacts with: Mouse

Product name

Anti-VE Cadherin antibody [EPR18229]
See all VE Cadherin primary antibodies
Description

Rabbit monoclonal [EPR18229] to VE Cadherin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lung, placenta, heart, kidney and spleen lysates; bEnd.3 whole cell lysate. ICC/IF: bEnd.3 cells. IP: Mouse lung whole cell lysate; bEnd.3 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18229
Isotype

IgG
Research areas

Western blot - Anti-VE Cadherin antibody [EPR18229] (ab205336)

All lanes : Anti-VE Cadherin antibody [EPR18229] (ab205336) at 1/1000 dilution

Lane 1 : Mouse lung lysate
Lane 2 : Mouse placenta lysate
Lane 3 : bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 88 kDa
Observed band size: 125,90 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] (ab205336)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (Mouse brain microvascular endothelial cell line) cells labeling VE Cadherin with ab205336 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing membrane staining on bEnd.3 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody -Loading control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594 ) preadsorbed (ab150120) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205336 at 1/1000 dilution followed by ab150120 at 1/500 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/500 dilution.
Western blot - Anti-VE Cadherin antibody [EPR18229] (ab205336)

All lanes : Anti-VE Cadherin antibody [EPR18229] (ab205336) at 1/1000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 88 kDa
Observed band size: 120,90 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] (ab205336)

VE Cadherin was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Mouse lung whole cell lysate 10ug (Input).
Lane 2: ab205336 IP in Mouse lung whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in Mouse lung whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.

Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] (ab205336)

VE Cadherin was immunoprecipitated from 1mg of bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate with ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: bEnd.3 whole cell lysate 10ug (Input).
Lane 2: ab205336 IP in bEnd.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Anti-VE Cadherin antibody [EPR18229] (ab205336)

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