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Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)

Key features and details

  • Mouse monoclonal [5D5] to N Cadherin - Intercellular Junction Marker
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker
    See all N Cadherin primary antibodies

  • Description

    Mouse monoclonal [5D5] to N Cadherin - Intercellular Junction Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human N Cadherin.

  • Positive control

    • A431, NIH-3T3, Hela, C6 and LNCap cells. Human lung cancer, colon cancer, ovarian cancer and mammary cancer tissue.

Properties

Images

  • Western blot - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Western blot - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    All lanes : Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : NIH-3T3 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : LNCap cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : HRP-Goat Anti-Mouse Igg (Fc) at 1/10000 dilution

    Predicted band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952) This image is courtesy of an anonymous Abreview

    ab98952 staining N Cadherin in human NSCLC tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with using HOPE technique and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952) This image is courtesy of an anonymous Abreview

    ab98952 staining N Cadherin in paraffin-embedded human lung cancer tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with HOPE and blocked. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A TRITC-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Flow Cytometry - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Overlay histogram showing HeLa cells stained with ab98952 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98952, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Immunohistochemical analysis of paraffin-embedded Human lung cancer (1), colon cancer (2), ovarian cancer (3) and mammary cancer (4), using ab98952 at 1/200 dilution with DAB staining.
  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
    Immunofluorescence analysis of A431 cells using ab98952 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye

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