Antibodies, Proteins, Kits and Reagents for Life Science

Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP90] to N Cadherin - BSA and Azide free
Suitable for: IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-N Cadherin antibody [SP90] - BSA and Azide free
See all N Cadherin primary antibodies
Description

Rabbit monoclonal [SP90] to N Cadherin - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, Flow Cytmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
Immunogen

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human mesothelioma, liver, adrenal gland fallopian tube, renal cell carcinoma, stomach and heart tissue; Mouse liver tissue; Rat liver tissue. Flow Cyt: HeLa cells.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab240403 is the carrier-free version of ab225719. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240403 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP90
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719)
Flow Cytometry - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) labeling N Cadherin with purified ab225719 at 1/20 dilution (4.55 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Formalin-fixed, paraffin-embedded human stomach tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).
Flow Cytometry - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling N Cadherin with ab225719 at 1/100 (green) compared with an isotype control rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Formalin-fixed, paraffin-embedded human liver tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab225719).
Anti-N Cadherin antibody [SP90] - BSA and Azide free (ab240403)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com