Anti-N Cadherin antibody [8C11] - BSA and Azide free (ab233767)
![Anti-N Cadherin antibody [8C11] - BSA and Azide free (ab233767)](https://www.abcam.com/ps/products/233/ab233767/Images/ab233767-332509-anti-n-cadherin-antibody-8c11-immunohistochemistry.jpg "Anti-N Cadherin antibody [8C11] - BSA and Azide free (ab233767)")
Key features and details
- Mouse monoclonal [8C11] to N Cadherin - BSA and Azide free
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-N Cadherin antibody [8C11] - BSA and Azide free
See all N Cadherin primary antibodies -
Description
Mouse monoclonal [8C11] to N Cadherin - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Fusion protein corresponding to Human N Cadherin (extracellular). A recombinant maltose binding protein fused to the extracellular domain of human N-cadherin.
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Positive control
- IHC-P: Human heart tissue. ICC/IF: SH-SY5Y cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
ab233767 is a PBS-only version of ab19348.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8C11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [8C11] - BSA and Azide free (ab233767)
IHC image of N cadherin staining in a section of formalin-fixed paraffin-embedded normal human heart performed on a Leica BONDTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19348, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This data was developed using the same antibody clone (ab19348) in a different buffer formulation.
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![Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] - BSA and Azide free (ab233767)](https://www.abcam.com/ps/products/233/ab233767/Images/ab233767-332510-anti-n-cadherin-antibody-8c11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] - BSA and Azide free (ab233767)N-Cadherin staining in SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells.
The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19348 at 5 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone (ab19348) in a different buffer formulation.
