Astana Biomed Group, an authorized Abcam distributor in Central Asia

APC Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab224960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • APC Rabbit monoclonal [EP700Y] to E Cadherin - Intercellular Junction Marker
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm

Overview

  • Product name

    APC Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker
    See all E Cadherin primary antibodies

  • Description

    APC Rabbit monoclonal [EP700Y] to E Cadherin - Intercellular Junction Marker

  • Host species

    Rabbit

  • Conjugation

    APC. Ex: 645nm, Em: 660nm

  • Specificity

    E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed. This antibody has been tested on human samples in both WB and IHC. Customer feedback (see Abreview) suggests the antibody does not perform well in IHC on mouse tissue.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: MCF7 cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - APC Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab224960)
    Flow Cytometry - APC Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab224960)

    Overlay histogram showing MCF7 cells stained with ab224960 (red line). The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224960, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

  • APC Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab224960)
    APC Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab224960)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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