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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins

Anti-E Cadherin antibody [EPR16845-108] (ab181296)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-E Cadherin antibody [EPR16845-108] (ab181296)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16845-108] to E Cadherin
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-E Cadherin antibody [EPR16845-108]
    See all E Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EPR16845-108] to E Cadherin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse E-Cadherin active protein (aa1-709); Rat spleen lysate; Mouse plasma and serum; Mouse brain lysate. IP: Mouse serum.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16845-108
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development
    • Signal Transduction
    • Antibodies
    • e cadherin

Images

  • Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296)
    Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296)
    Lane 1 : Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/20000 dilution
    Lane 2 : Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/100000 dilution

    All lanes : His-tagged mouse E-Cadherin active protein (aa1-709)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa
    Observed band size: 120,84 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds
  • Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296)
    Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296)
    All lanes : Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/1000 dilution

    Lane 1 : Rat spleen lysate
    Lane 2 : Mouse plasma
    Lane 3 : Mouse serum
    Lane 4 : Mouse brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 & 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa
    Observed band size: 120,84 kDa why is the actual band size different from the predicted?



     

    Exposure time : Lanes 1-2: 30 seconds; Lanes 3-4: 15 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 11076937; PMID: 11953314).

  • Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (ab181296)
    Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (ab181296)

    E Cadherin was immunoprecipitated from 1 mg of mouse serum with ab181296 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse serum 10 µg (Input). 

    Lane 2: ab181296 IP in mouse serum. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181296 in mouse serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-E Cadherin antibody [EPR16845-108] (ab181296)
    Anti-E Cadherin antibody [EPR16845-108] (ab181296)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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