Anti-E Cadherin antibody [SP64] - BSA and Azide free (ab240984)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP64] to E Cadherin - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-E Cadherin antibody [SP64] - BSA and Azide free
See all E Cadherin primary antibodies -
Description
Rabbit monoclonal [SP64] to E Cadherin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Recombinant fragment within Human E Cadherin aa 600-750. The exact sequence is proprietary.
Database link: P12830 -
Positive control
- IHC-P: Human breast carcinoma, skin squamous cell carcinoma, skin and prostate tissues.
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab240984 is the carrier-free version of ab227639. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240984 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP64 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling E Cadherin with ab227639 at 1/100 dilution (1.22 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227639 for 10 mins at room temperature.This image was generated using ab227639, the same clone, but with a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling E Cadherin with ab227639 at 1/100 dilution (1.22 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human liver, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227639 for 10 mins at room temperature.This image was generated using ab227639, the same clone, but with a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling E Cadherin with ab227639 at 1/100 dilution (1.22 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227639 for 10 mins at room temperature.This image was generated using ab227639, the same clone, but with a different buffer formulation.
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Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for E Cadherin using ab227639 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227639)
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