Anti-14-3-3 gamma/YWHAG antibody (ab237732)
Key features and details
- Rabbit polyclonal to 14-3-3 gamma/YWHAG
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-14-3-3 gamma/YWHAG antibody
See all 14-3-3 gamma/YWHAG primary antibodies -
Description
Rabbit polyclonal to 14-3-3 gamma/YWHAG -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Orange
-
Immunogen
Recombinant fragment corresponding to Human 14-3-3 gamma/YWHAG aa 100-200.
Database link: P61981 -
Positive control
- WB: Mouse brain lysate; Rat brain lysate; NIH/3T3, A431, K562, U-251 MG, HeLa and Jurkat whole cell lysates; Human placenta lysate. IHC-P: Human ovarian cancer and pancreatic cancer tissues. IP: NIH/3T3 whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab237732)All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab237732) at 1/500 dilution
Lane 1 : Mouse brain lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Human placenta lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
-
Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab237732)All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab237732) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 6 : Rat brain lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 gamma/YWHAG antibody (ab237732)Paraffin-embedded human ovarian cancer tissue stained for 14-3-3 gamma/YWHAG using ab237732 at 1/100 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 gamma/YWHAG antibody (ab237732)Paraffin-embedded human pancreatic cancer tissue stained for 14-3-3 gamma/YWHAG using ab237732 at 1/100 dilution in immunohistochemical analysis.
-
Immunoprecipitation - Anti-14-3-3 gamma/YWHAG antibody (ab237732)14-3-3 gamma/YWHAG was immunoprecipitated from 0.5 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab237732 at 1/200 dilution.
Lane 1: Control rabbit IgG IP in NIH/3T3 whole cell lysate.
Lane 2: ab237732 IP in NIH/3T3 whole cell lysate.
Lane 3: NIH/3T3 whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
