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Signal Transduction Growth Factors/Hormones FGF

Anti-FRS2 antibody [EPR14724] (ab183492)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-FRS2 antibody [EPR14724] (ab183492)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14724] to FRS2
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-FRS2 antibody [EPR14724]
    See all FRS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14724] to FRS2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, HeLa and SH-SY5Y lysates; SH-SY5Y cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14724
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • FGF

Images

  • Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)
    Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)
    All lanes : Anti-FRS2 antibody [EPR14724] (ab183492) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

  • Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)
    Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)
    Anti-FRS2 antibody [EPR14724] (ab183492) at 1/2000 dilution + SH-SY5Y cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

  • Flow Cytometry - Anti-FRS2 antibody [EPR14724] (ab183492)
    Flow Cytometry - Anti-FRS2 antibody [EPR14724] (ab183492)

    Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling FRS2 with ab183492 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-FRS2 antibody [EPR14724] (ab183492)
    Immunoprecipitation - Anti-FRS2 antibody [EPR14724] (ab183492)

    Western blot analysis of FRS2 in Human fetal brain lysate immunoprecipitated using ab183492 at 1/50 dilution (Lane 1). Lane 2: TBS instead of Human fetal brain lysate.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-FRS2 antibody [EPR14724] (ab183492)
    Anti-FRS2 antibody [EPR14724] (ab183492)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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