Anti-FRS2 (phospho Y436) antibody (ab193363)
Key features and details
- Rabbit polyclonal to FRS2 (phospho Y436)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FRS2 (phospho Y436) antibody
See all FRS2 primary antibodies -
Description
Rabbit polyclonal to FRS2 (phospho Y436) -
Host species
Rabbit -
Specificity
ab193363 detects endogenous levels of FRS2 only when phosphorylated at tyrosine 436. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human FRS2 (phospho Y436) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q8WU20 -
Positive control
- Human brain tissue, extracts from HuvEc cells or Jurkat cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab193363 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRS2 (phospho Y436) antibody (ab193363)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling FRS2 with ab193363 at a 1/50 dilution. Right panel has been pre-incubated with blocking peptide.
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Western blot - Anti-FRS2 (phospho Y436) antibody (ab193363)All lanes : Anti-FRS2 (phospho Y436) antibody (ab193363) at 1/500 dilution
Lane 1 : HuvEc extract with Antigen specific peptide
Lane 2 : Huvec extract
Lane 3 : Jurkat extract
Predicted band size: 65 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRS2 (phospho Y436) antibody (ab193363) This image is courtesy of an anonymous Abreviewab193363 staining FRS2 (phospho Y436) in human lung cancer sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde. Antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/100 ) at 4°C for 16 hours. An HRP-conjugated Goat anti-rabbit polyclonal (1/250) was used as the secondary antibody.
