Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9654(B)] to 14-3-3 gamma/YWHAG
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)]
See all 14-3-3 gamma/YWHAG primary antibodies
Description

Rabbit monoclonal [EPR9654(B)] to 14-3-3 gamma/YWHAG
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

corresponding to Human 14-3-3 gamma/YWHAG aa 50-150.
Database link: P61981
Positive control
- WB: HEK293T, A431, HeLa, K562 and A549 cell lysates; Human fetal heart lysate.
General notes
This product was previously labelled as 14-3-3 gamma

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9654(B)
Isotype

IgG
Research areas

Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048)

All lanes : Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : YWHAG knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 28 kDa
Observed band size: 28 kDa

Lanes 1-4: Merged signal (red and green). Green - ab137048 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.

ab137048 Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] was shown to specifically react with 14-3-3 gamma/YWHAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266734 (knockout cell lysate ab257805) was used. Wild-type and 14-3-3 gamma/YWHAG knockout samples were subjected to SDS-PAGE. ab137048 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048)

All lanes : Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048) at 1/1000 dilution

Lane 1 : HeLa lysate
Lane 2 : K562 lysate
Lane 3 : A549 lysate
Lane 4 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 28 kDa
Anti-14-3-3 gamma/YWHAG antibody [EPR9654(B)] (ab137048)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com